RESEARCH PROJECTS

Our Current Focus

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  • Generation of patient-derived 3D bladder organoids: Modelling and molecular characterization of bladder cancer pathogenesis in patient-derived organoids

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  • Establishment of patient-derived tumoroids from urine samples: We isolate cells from urine samples and seed them in 3D cultures containing bladder expansion media to expand the patient bladder cells in organoid –tumoroid structure

  • Unbiased approach:

    • Proteomics and genomics analysis of treatment naive high grade NMIBC-derived tumoroids and healthy tissue-derived organoids

    • Platform for anti-bladder cancer drug screening and drug-induced toxicity

  • Establishment of co-culture system of patient-derived bladder cancer tumoroids with autologous PBMCs/ CD8+T cells

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  • Candidate approach:

    • Molecular characterization and pharmacological targeting of ARID1A deficiency in bladder cancer

    • Generation of transgenic patient-derived bladder organoid lines to examine the molecular mechanisms of tumorigenesis

    • Induced Cell Death (ICD) strategy towards elimination of bladder cancer

    • Characterization of aminopeptidase NPEPPS as a target to overcome gemcitabine/cisplatin resistance in Patient-derived bladder cancer organoids. Jones et al 2021, bioRxiv

 
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TECHNICAL EXPERTISE

PATIENT-DERIVED LIVER ORGANOIDS

PROTEOMICS AND GENOMICS

DRUG SCREENING AND DRUG TOXICITY PLATFORM

CONFOCAL MICROSCOPY